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GREAT AT SMALL THINGS

Sequence Manipulation Suite

Restriction Enzymes


A Ba - Bp Bsa - Bsl Bsm - Bss Bst - Bve C - E F - L M - N O - Q R - S T - Z

EnzymeRestriction siteOriginTemperature
Cac8I...GCN NGC...
...CGN NCG...
Clostridium acetobutylicum37 oC
CaiI...CAGNNN CTG...
...GTC NNNGAC...
Delftia acidovorans60 oC
CciNI...GC GGCCGC...
...CGCCGG CG...
Curtobacterium citreum65 oC
CelII...GC TNAGC...
...CGANT CG...
Coccochloris elabens65 oC
CfoI...GCG C...
...C GCG...
Clostridium formicoaceticum37 oC
Cfr9I...C CCGGG...
...GGGCC C...
Citrobacter freundii37 oC
Cfr10I...R CCGGY...
...TGGCC R...
Citrobacter freundii37 oC
Cfr13I...G GNCC...
...CCNG G...
Citrobacter freundii37 oC
Cfr42I...CCGC GG...
...GG CGCC...
Citrobacter freundii37 oC
CfrI...Y GGCCR...
...RCCGG Y...
Citrobacter freundii65 oC
ClaI...AT CGAT...
...TAGC TA...
Caryophanon latum65 oC
CpoI...CG GWCCG...
...GCCWG GC...
Corynebacterium variabile55 oC
CseI...GACGCNNNNN NNNNNN...
...CTGCGNNNNNNNNNN N...
Chryseobacterium species65 oC
CspI...CG GWCCG...
...GCCWG GC...
Clostridium species60 oC
Csp45I...TT CGAA...
...AAGC TT...
Clostridium sporogenes60 oC
Csp6I...G TAC...
...CAT G...
Corynebacterium species65 oC
CspAI...A CCGGT...
...TGGCC A...
Corynebacterium species65 oC
CviAII...C ATG...
...GTA C...
Chlorella virus60 oC
CviJI...RG CY...
...YC GR...
Chlorella strain65 oC
DdeI...C TNAG...
...GANT C...
Desulfovibrio desulfuricans50 oC
DpnI...GmeA TC...
...CT meAG...
Streptococcus pneumoniae37 oC
DpnII...N GATCN...
...NCTAG N...
Streptococcus pneumoniae65 oC
DraI...TTT AAA...
...AAA TTT...
Deinococcus radiophilus50 oC
DraII...RG GNCCY...
...TCCNG GR...
Deinococcus radiophilus55 oC
DraIII...CACNNN GTG...
...GTG NNNCAC...
Deinococcus radiophilus60 oC
DrdI...GACNNNN NNGTC...
...CTGNN NNNNCAG...
Deinococcus radiophilus37 oC
DriI...GACNNN NNGTC...
...CTGNN NNNCAG...
Deinococcus radiophilus60 oC
DseDI...GACNNNN NNGTC...
...CTGNN NNNNCAG...
Deinococcus species60 oC
EaeI...Y GGCCR...
...RCCGG Y...
Enterobacter aerogenes65 oC
EagI...C GGCCG...
...GCCGG C...
Pantoea agglomerans55 oC
Eam1104I...CTCTTCN NNNN...
...GAGAAGNNNN N...
Enterobacter amnigenus60 oC
Eam1105I...GACNNN NNGTC...
...CTGNN NNNCAG...
Enterobacter amnigenus37 oC
EarI...CTCTTCN NNNN...
...GAGAAGNNNN N...
Enterobacter aerogenes60 oC
EciI...GGCGGANNNNNNNNNNN N...
...CCGCCTNNNNNNNNN NNN...
Escherichia coli55 oC
Ecl136II...GAG CTC...
...CTC GAG...
Enterobacter cloacae55 oC
EclHKI...GACNNN NNGTC...
...CTGNN NNNCAG...
Enterobacter cloacae50 oC
EclXI...C GGCCG...
...GCCGG C...
Enterobacter cloacae60 oC
Eco24I...GRGCY C...
...C TCGRG...
Escherichia coli60 oC
Eco31I...GGTCTCN NNNNN...
...CCAGAGNNNNN N...
Escherichia coli50 oC
Eco32I...GAT ATC...
...CTA TAG...
Escherichia coli37 oC
Eco47I...G GWCC...
...CCWG G...
Escherichia coli37 oC
Eco47III...AGC GCT...
...TCG CGA...
Escherichia coli37 oC
Eco52I...C GGCCG...
...GCCGG C...
Escherichia coli37 oC
Eco57I...CTGAAGNNNNNNNNNNNNNNNN N...
...GACTTCNNNNNNNNNNNNNN NNN...
Escherichia coli37 oC
Eco57MI...CTGRAGNNNNNNNNNNNNNNNN N...
...GACYTCNNNNNNNNNNNNNN NNN...
Escherichia coli37 oC
Eco72I...CAC GTG...
...GTG CAC...
Escherichia coli60 oC
Eco81I...CC TNAGG...
...GGANT CC...
Escherichia coli60 oC
Eco88I...C YCGRG...
...GRGCY C...
Escherichia coli37 oC
Eco91I...G GTNACC...
...CCANTG G...
Escherichia coli37 oC
Eco105I...TAC GTA...
...ATG CAT...
Escherichia coli37 oC
Eco130I...C CWWGG...
...GGWWC C...
Escherichia coli37 oC
Eco147I...AGG CCT...
...TCC GGA...
Escherichia coli37 oC
EcoICRI...GAG CTC...
...CTC GAG...
Escherichia coli37 oC
EcoNI...CCTNN NNNAGG...
...GGANNN NNTCC...
Escherichia coli37 oC
EcoO109I...RG GNCCY...
...YCCNG GR...
Escherichia coli37 oC
EcoO65I...G GTNACC...
...CCANTG G...
Escherichia coli37 oC
EcoP15I...CAGCAGNNNNNNNNNNNNNNNNNNNNNNNNN NNN...
...GTCGTCNNNNNNNNNNNNNNNNNNNNNNNNNNN N...
Escherichia coli37 oC
EcoRI...G AATTC...
...CTTAA G...
Escherichia coli37 oC
EcoRII...N CCWGGN...
...NGGWCC N...
Escherichia coli37 oC
EcoR V...GAT ATC...
...CTA TAG...
Escherichia coli37 oC
EcoT14I...C CWWGG...
...GGWWC C...
Escherichia coli37 oC
EcoT22I...ATGCA T...
...T ACGTA...
Escherichia coli37 oC
EcoT38I...GRGCY C...
...C YCGRG...
Escherichia coli37 oC
EgeI...GGC GCC...
...CCG CGG...
Enterobacter gergoviae37 oC
EheI...GGC GCC...
...CCG CGG...
Pantoea agglomerans37 oC
ErhI...C CWWGG...
...GGWWC C...
Erwinia rhapontici37 oC
Esp3I...CGTCTCN NNNNN...
...GCAGAGNNNNN N...
Erwinia species37 oC