| Enzyme | Restriction site | Origin | Temperature |
|---|
| BsmI | ...GAATGCN N...
...CTTAC GNN... | Geobacillus stearothermophilus | 65 oC |
| BsmAI | ...GTCTCN NNNNN...
...CAGAGNNNNN N... | Geobacillus stearothermophilus | 55 oC |
| BsmBI | ...CGTCTCN NNNNN...
...GCAGAGNNNNN N... | Geobacillus stearothermophilus | 55 oC |
| BsmFI | ...GGGACNNNNNNNNNN NNNNN...
...CCCTGNNNNNNNNNNNNNN N... | Geobacillus stearothermophilus | 65 oC |
| Bso31I | ...GGTCTCN NNNNN...
...CCAGAGNNNNN N... | Geobacillus stearothermophilus | 55 oC |
| BsoBI | ...C YCGRG...
...GRGCY C... | Geobacillus stearothermophilus | 37 oC |
| Bsp119I | ...TT CGAA...
...AAGC TT... | Bacillus species | 37 oC |
| Bsp120I | ...G GGCCC...
...CCCGG G... | Bacillus species | 37 oC |
| Bsp1286I | ...GDGCH C...
...C HCGDG... | Bacillus sphaericus | 37 oC |
| Bsp13I | ...T CCGGA...
...AGGCC T... | Bacillus species | 50 oC |
| Bsp1407I | ...T GTACA...
...ACATG T... | Geobacillus stearothermophilus | 37 oC |
| Bsp143I | ...N GATCN...
...NCTAG N... | Bacillus species | 37 oC |
| Bsp143II | ...RGCGC Y...
...Y CGCGR... | Bacillus species | 37 oC |
| Bsp1720I | ...GC TNAGC...
...CGANT CG... | Bacillus species | 37 oC |
| Bsp19I | ...C CATGG...
...GGTAC C... | Bacillus species | 37 oC |
| Bsp68I | ...TCG CGA...
...AGC GCT... | Bacillus species | 37 oC |
| BspANI | ...GG CC...
...CC GG... | Bacillus species | 60 oC |
| BspDI | ...AT CGAT...
...TAGC TA... | Bacillus species | 37 oC |
| BspEI | ...T CCGGA...
...AGGCC T... | Bacillus species | 37 oC |
| BspHI | ...T CATGA...
...AGTAC T... | Bacillus species | 37 oC |
| BspLI | ...GGN NCC...
...CCN NGG... | Bacillus species | 37 oC |
| BspLU11I | ...A CATGT...
...TGTAC A... | Bacillus species | 48 oC |
| BspMI | ...ACCTGCNNNN NNNNN...
...TGGACGNNNNNNNN N... | Geobacillus stearothermophilus | 37 oC |
| BspMAI | ...CTGCA G...
...C ACGTC... | Bacillus species | 65 oC |
| BspPI | ...GGATCNNNN NN...
...CCTAGNNNNN N... | Bacillus species | 55 oC |
| BspTI | ...C TTAAG...
...GAATT C... | Bacillus species | 37 oC |
| BspT104I | ...TT CGAA...
...AAGC TT... | Bacillus species | 37 oC |
| BspT107I | ...G GYRCC...
...CCRYG G... | Bacillus species | 37 oC |
| BspTNI | ...GGTCTCN NNNNN...
...CCAGAGNNNNN N... | Bacillus species | 37 oC |
| BspXI | ...AT CGAT...
...TAGC TA... | Bacillus sphaericus | 37 oC |
| BsrI | ...ACTGGN N...
...TGAC CNN... | Geobacillus stearothermophilus | 65 oC |
| BsrBI | ...CCG CTC...
...GGC GAG... | Geobacillus stearothermophilus | 37 oC |
| BsrDI | ...GCAATGNN N...
...CGTTAC NNN... | Geobacillus stearothermophilus | 65 oC |
| BsrFI | ...R CCGGY...
...YGGCC R... | Geobacillus stearothermophilus | 37 oC |
| BsrGI | ...T GTACA...
...ACATG T... | Geobacillus stearothermophilus | 37 oC |
| BsrSI | ...ACTGGN N...
...TGAC CNN... | Geobacillus stearothermophilus | 65 oC |
| BssAI | ...R CCGGY...
...TGGCC R... | Bacillus species | 65 oC |
| BssECI | ...C CNNGG...
...GGNNC C... | Geobacillus stearothermophilus | 60 oC |
| BssHII | ...G CGCGC...
...CGCGC G... | Geobacillus stearothermophilus | 50 oC |
| BssKI | ...N CCNGGN...
...NGGNCC N... | Geobacillus stearothermophilus | 60 oC |
| BssNAI | ...GTA TAC...
...CAT ATG... | Geobacillus stearothermophilus | 37 oC |
| BssSI | ...C ACGAG...
...GTGCT C... | Geobacillus stearothermophilus | 37 oC |
| BssT1I | ...C CWWGG...
...GGWWC C... | Geobacillus stearothermophilus | 60 oC |
