| Enzyme | Restriction site | Origin | Temperature |
|---|
| RcaI | ...T CATGA...
...AGTAC T... | Rhodococcus capsulatum | 37 oC |
| RsaI | ...GT AC...
...CA TG... | Rhodobacter sphaeroides | 37 oC |
| RsrII | ...CG GWCCG...
...GCCWG GC... | Rhodobacter sphaeroides | 37 oC |
| Rsr2I | ...CG GWCCG...
...GCCWG GC... | Rhodobacter sphaeroides | 37 oC |
| SacI | ...GAGCT C...
...C TCGAG... | Streptomyces achromogenes | 37 oC |
| SacII | ...CCGC GG...
...GG CGCC... | Streptomyces achromogenes | 37 oC |
| SalI | ...G TCGAC...
...CAGCT G... | Streptomyces albus | 37 oC |
| SapI | ...GCTCTTCN NNNN...
...CGAGAAGNNNN N... | Saccharopolyspora species | 37 oC |
| SatI | ...GC NGC...
...CGN CG... | Staphylococcus arlettae | 37 oC |
| Sau3AI | ...N GATCN...
...NCTAG N... | Staphylococcus aureus | 37 oC |
| Sau96I | ...G GNCC...
...CCNG G... | Staphylococcus aureus | 37 oC |
| SbfI | ...CCTGCA GG...
...GG ACGTCC... | Streptomyces species | 37 oC |
| ScaI | ...AGT ACT...
...TCA TGA... | Streptomyces caespitosus | 37 oC |
| SchI | ...GAGTCNNNNN N...
...CTCAGNNNNN N... | Staphylococcus cohnii | 37 oC |
| ScrFI | ...CC NGG...
...GGN CC... | Salmonella typhi | 37 oC |
| SdaI | ...CCTGCA GG...
...GG ACGTCC... | Streptomyces diastaticus | 37 oC |
| SduI | ...GDGCH C...
...C HCGDG... | Enterococcus durans | 37 oC |
| SexAI | ...A CCWGGT...
...TGGWCC A... | Streptomyces exfoliatus | 37 oC |
| SfaNI | ...GCATCNNNNN NNNNN...
...CGTAGNNNNNNNNN N... | Enterococcus faecalis | 37 oC |
| SfcI | ...C TRYAG...
...GAYRT C... | Enterococcus faecium | 25 oC |
| SfiI | ...GGCCNNNN NGGCC...
...CCGGN NNNNCCGG... | Streptomyces fimbriatus | 50 oC |
| SfoI | ...GGC GCC...
...CCG CGG... | Serratia fonticola | 37 oC |
| Sfr274I | ...C TCGAG...
...GAGCT C... | Streptomyces fradiae | 50 oC |
| Sfr303I | ...CCGC GG...
...GG CGCC... | Streptomyces fradiae | 37 oC |
| SfuI | ...TT CGAA...
...AAGC TT... | Streptomyces fulvissimus | 37 oC |
| SgfI | ...GCGAT CGC...
...CGC TAGCG... | Streptomyces griseoruber | 37 oC |
| SgrAI | ...CR CCGGYG...
...GYGGCC RC... | Streptomyces griseus | 37 oC |
| SgrBI | ...CCGC GG...
...GG CGCC... | Streptomyces griseus | 37 oC |
| SgsI | ...GG CGCGCC...
...CCGCGC GG... | Streptomyces griseus | 37 oC |
| SinI | ...G GWCC...
...CCWG G... | Salmonella enterica | 37 oC |
| SlaI | ...C TCGAG...
...GAGCT C... | Streptomyces lavendulae | 37 oC |
| SmaI | ...CCC GGG...
...GGG CCC... | Serratia marcescens | 25 oC |
| SmiI | ...ATTT AAAT...
...TAAA TTTA... | Streptococcus milleri | 37 oC |
| SmiMI | ...CAYNN NNRTG...
...GTRNN NNYAC... | Flavobacterium mizutaii | 37 oC |
| SmlI | ...C TYRAG...
...GARYT C... | Stenotrophomonas maltophilia | 55 oC |
| SmuI | ...CCCGCNNNN NNN...
...GGGCGNNNNNN N... | Sphingobacterium multivorum | 37 oC |
| SnaBI | ...TAC GTA...
...ATG CAT... | Sphaerotilus natans | 37 oC |
| SpeI | ...A CTAGT...
...TGATC A... | Sphaerotilus natans | 37 oC |
| SphI | ...GCATG C...
...C GTACG... | Streptomyces phaeochromogenes | 37 oC |
| Sse8387I | ...CCTGCA GG...
...GG ACGTCC... | Streptomyces species | 37 oC |
| Sse9I | ...N AATTN...
...NTTAA N... | Sporosarcina species | 55 oC |
| SseBI | ...AGG CCT...
...TCC GGA... | Streptomyces species | 37 oC |
| SsiI | ...C CGC...
...GGC G... | Staphylococcus sciuri | 37 oC |
| SspI | ...AAT ATT...
...TTA TAA... | Sphaerotilus species | 37 oC |
| SspBI | ...T GTACA...
...ACATG T... | Streptomyces species | 37 oC |
| SstI | ...GAGCT C...
...C TCGAG... | Streptomyces stanford | 37 oC |
| SstII | ...CCGC GG...
...GG CGGC... | Streptomyces stanford | 37 oC |
| StrI | ...C TCGAG...
...TAGCT C... | Streptomyces thermodiastaticus | not known |
| StuI | ...AGG CCT...
...TCC GGA... | Streptomyces tubercidicus | 37 oC |
| StyI | ...C CWWGG...
...GGWWC C... | Salmonella typhi | 37 oC |
| StyD4I | ...N CCNGGN...
...NGGNCC N... | Salmonella typhi | 37 oC |
| SwaI | ...ATTT AAAT...
...TAAA TTTA... | Staphylococcus warneri | 25 oC |
