| Enzyme | Restriction site | Origin | Temperature |
|---|
| MabI | ...A CCWGGT...
...TGGECC A... | Microbacterium arborescens | 37 oC |
| MaeI | ...C TAG...
...GAT C... | Methanococcus aeolicus | 37 oC |
| MaeII | ...A CGT...
...TGC A... | Methanococcus aeolicus | 50 oC |
| MaeIII | ...N GTNACN...
...NCANTG N... | Methanococcus aeolicus | 55 oC |
| MamI | ...GATNN NNATC...
...CTANN NNTAG... | Microbacterium ammoniaphilum | 37 oC |
| MbiI | ...CCG CTC...
...GGC GAG... | Moraxella bovis | 37 oC |
| MboI | ...N GATCN...
...NCTAG N... | Moraxella bovis | 37 oC |
| MboII | ...GAAGANNNNNNNN N...
...CTTCTNNNNNNN NN... | Moraxella bovis | 37 oC |
| MfeI | ...C AATTG...
...GTTAA C... | Mycoplasma fermentans | 37 oC |
| MflI | ...R GATCY...
...TCTAG R... | Corynebacterium flavescens | 37 oC |
| MhlI | ...GDGCH C...
...C HCGDG... | Nesterenkonia halobia | 37 oC |
| MlsI | ...TGG CCA...
...ACC GGT... | Micrococcus luteus | 37 oC |
| MluI | ...A CGCGT...
...TGCGC A... | Micrococcus luteus | 37 oC |
| MluNI | ...TGG CCA...
...ACC GGT... | Micrococcus luteus | 37 oC |
| Mly113I | ...GG CGCC...
...CCGC GG... | Micrococcus lylae | 37 oC |
| MlyI | ...GAGTCNNNNN N...
...CTCAGNNNNN N... | Micrococcus lylae | 37 oC |
| MmeI | ...TCCRACNNNNNNNNNNNNNNNNNNNN N...
...AGGRTGNNNNNNNNNNNNNNNNNN NNN... | Methylophilus methylotrophus | 37 oC |
| MnlI | ...CCTCNNNNNNN N...
...GGAGNNNNNN NN... | Moraxella nonliquefaciens | 37 oC |
| Mph1103I | ...ATGCA T...
...T ACGTA... | Psychrobacter phenylpyruvicus | 37 oC |
| MroI | ...T CCGGA...
...AGGCC T... | Kocuria rosea | 37 oC |
| MroNI | ...G CCGGC...
...CGGCC G... | Kocuria rosea | 37 oC |
| MroXI | ...GAANN NNTTC...
...CTTNN NNAAG... | Kocuria rosea | 37 oC |
| MscI | ...TGG CCA...
...ACC GGT... | Micrococcus species | 37 oC |
| MseI | ...T TAA...
...AAT T... | Micrococcus species | 37 oC |
| MslI | ...CAYNN NNRTG...
...GTRNN NNYAC... | Moraxella osloensis | 37 oC |
| MspCI | ...C TTAAG...
...GAATT C... | Micrococcus species | 37 oC |
| MspI | ...C CGG...
...GGC C... | Moraxella species | 37 oC |
| Msp20I | ...TGG CCA...
...ACC GGT... | Micrococcus species | 37 oC |
| MspA1I | ...CMG CKG...
...GKC GMC... | Moraxella species | 37 oC |
| MspR9I | ...CC NGG...
...GGN CC... | Micrococcus species | 37 oC |
| MssI | ...GTTT AAAC...
...CAAA TTTG... | Methylobacterium species | 37 oC |
| MunI | ...C AATTG...
...GTTAA C... | Mycoplasma species | 37 oC |
| MvaI | ...CC WGG...
...GGW CC... | Kocuria varians | 37 oC |
| Mva1269I | ...GAATGCN N...
...CTTAC GNN... | Kocuria varians | 37 oC |
| MvnI | ...CG CG...
...GC GC... | Kocuria varians | 37 oC |
| MvrI | ...CGAT CG...
...GC TAGC... | Kocuria varians | not known |
| MwoI | ...GCNNNNN NNGC...
...CGNN NNNNNCG... | Methanothermobacter wolfeii | 60 oC |
| NaeI | ...GCC GGC...
...CGG CCG... | Lechevalieria aerocolonigenes | 37 oC |
| NarI | ...GG CGCC...
...CCGC GG... | Nocardia argentinensis | 37 oC |
| NciI | ...CC SGG...
...GGS CC... | Neisseria cinerea | 37 oC |
| NcoI | ...C CATGG...
...GGTAC C... | Gordonia rubripertincta | 37 oC |
| NdeI | ...CA TATG...
...GTAT AC... | Neisseria denitrificans | 37 oC |
| NdeII | ...N GATCN...
...NCTAG N... | Neisseria denitrificans | 37 oC |
| NgoMIV | ...G CCGGC...
...CGGCC G... | Neisseria gonorrhoeae | 37 oC |
| NheI | ...G CTAGC...
...CGATC G... | Neisseria mucosa heidelbergensis | 37 oC |
| NlaIII | ...NCATG N...
...N GTACN... | Neisseria lactamica | 37 oC |
| NlaIV | ...GGN NCC...
...CCN NGG... | Neisseria lactamica | 37 oC |
| NmuCI | ...N GTSACN...
...NCTSTG N... | Neisseria mucosa | 37 oC |
| NotI | ...GC GGCCGC...
...CGCCGG CG... | Nocardia otitidiscaviarum | 37 oC |
| NruI | ...TCG CGA...
...AGC GCT... | Nocardia rubra | 37 oC |
| NsbI | ...TGC GCA...
...ACG CGT... | Neisseria subflava | 37 oC |
| NsiI | ...ATGCA T...
...T ACGTA... | Neisseria sicca | 37 oC |
| NspI | ...RCATG Y...
...Y GTACR... | Nostoc species | 37 oC |
| Nsp V | ...TT CGAA...
...AAGC TT... | Nostoc species | 37 oC |
