| Enzyme | Restriction site | Origin | Temperature |
|---|
| FalI | ...NNNNNN NNNNNNNNAAGNNNNNCTTNNNNNNNNNNNNN N...
...N NNNNNNNNNNNNNTTCNNNNNGAANNNNNNNN NNNNNN... | Flavobacterium aquatile | 37 oC |
| FaqI | ...GGGACNNNNNNNNNN NNNNN...
...CCCTGNNNNNNNNNNNNNN N... | Flavobacterium aquatile | 37 oC |
| FatI | ...N CATGN...
...NGTAC N... | Flavobacterium aquatile | 55 oC |
| FauI | ...CCCGCNNNN NNN...
...GGGCGNNNNNN N... | Flavobacterium aquatile | 55 oC |
| FauNDI | ...CA TATG...
...GTAT AC... | Flavobacterium aquatile | 37 oC |
| FbaI | ...T GATCA...
...ACTAG T... | Flavobacterium aquatile | 37 oC |
| FblI | ...GT MKAC...
...CAKM TG... | Flavobacterium balustinum | 55 oC |
| Fnu4HI | ...GC NGC...
...CGN CG... | Fusobacterium nucleatum | 37 oC |
| FokI | ...GGATGNNNNNNNNN NNNNN...
...CCTACNNNNNNNNNNNNN N... | Planomicrobium okeanokoites | 37 oC |
| FriOI | ...GRGCY C...
...C YCGRG... | Flavobacterium species | 37 oC |
| FseI | ...GGCCGG CC...
...CC GGCCGG... | Frankia species | 37 oC |
| FspI | ...TGC GCA...
...ACG CGT... | Fischerella species | 37 oC |
| FspAI | ...RTGC GCAY...
...TACG CGTR... | Flexibacter species | 37 oC |
| FspBI | ...C TAG...
...GAT C... | Flavobacterium species | 37 oC |
| Fsp4HI | ...GC NGC...
...CGN CG... | Flavobacterium species | 37 oC |
| GsuI | ...CTGGAGNNNNNNNNNNNNNNNN N...
...GACCTCNNNNNNNNNNNNNN NNN... | Gluconobacter suboxydans | 30 oC |
| HaeII | ...RGCGC Y...
...Y CGCGR... | Haemophilus aegyptius | 37 oC |
| HaeIII | ...GC CC...
...CC GG... | Haemophilus aegyptius | 37 oC |
| HapII | ...C CGG...
...GGC C... | Haemophilus aphrophilus | 37 oC |
| HgaI | ...GACGCNNNNNN NNNNN...
...CTGCGNNNNNNNNNN N... | Avibacterium paragallinarum | 37 oC |
| HhaI | ...GCG C...
...C GCG... | Haemophilus haemolyticus | 37 oC |
| Hin1I | ...GR CGYC...
...CYGC RG... | Haemophilus influenzae | 37 oC |
| Hin1II | ...NCATG N...
...N GTACN... | Haemophilus influenzae | 37 oC |
| Hin6I | ...G CGC...
...CGC G... | Haemophilus influenzae | 37 oC |
| HincII | ...GTY RAC...
...CAR YTG... | Haemophilus influenzae | 37 oC |
| HindII | ...GTY RAC...
...CAR YTR... | Haemophilus influenzae | 37 oC |
| HindIII | ...A AGCTT...
...TTCGA A... | Haemophilus influenzae | 37 oC |
| HinfI | ...G ANTC...
...CTNA G... | Haemophilus influenzae | 37 oC |
| HinP1I | ...G CGC...
...CGC G... | Haemophilus influenzae | 37 oC |
| HpaI | ...GTT AAC...
...CAA TTG... | Haemophilus parainfluenzae | 37 oC |
| HpaII | ...C CGG...
...GGC C... | Haemophilus parainfluenzae | 37 oC |
| HphI | ...GGTGANNNNNNNN N...
...CCACTNNNNNNN NN... | Haemophilus parahaemolyticus | 37 oC |
| Hpy188I | ...TCN GA...
...AG NCT... | Helicobacter pylori | 37 oC |
| Hpy188III | ...TC NNGA...
...AGNN CT... | Helicobacter pylori | 37 oC |
| Hpy8I | ...GTN NAC...
...CAN NTG... | Helicobacter pylori | 37 oC |
| Hpy99I | ...NCGWCG N...
...N GCWGCN... | Helicobacter pylori | 37 oC |
| HpyCH4III | ...ACN GT...
...TG NCA... | Helicobacter pylori | 37 oC |
| HpyCH4IV | ...A CGT...
...TGC A... | Helicobacter pylori | 37 oC |
| HpyCH4V | ...TG CA...
...AC GT... | Helicobacter pylori | 37 oC |
| HpyF3I | ...C TNAG...
...GANT C... | Helicobacter pylori | 37 oC |
| HpyF10VI | ...GCNNNNN NNGC...
...CGNN NNNNNCG... | Helicobacter pylori | 37 oC |
| Hsp92I | ...GR CGYC...
...CYCG RG... | Haemophilus species | 37 oC |
| Hsp92II | ...NCATG N...
...N GTACN... | Haemophilus species | 37 oC |
| HspAI | ...G CGC...
...CGC G... | Haemophilus species | 37 oC |
| ItaI | ...GC NGC...
...CGN CG... | Ilyobacter tartaricus | 37 oC |
| KasI | ...G GCGCC...
...CCGCG G... | Kluyvera ascorbata | 37 oC |
| KpnI | ...GGTAC C...
...C CATGG... | Klebsiella pneumoniae | 37 oC |
| Kpn2I | ...T CCGGA...
...AGGCC T... | Klebsiella pneumoniae | 55 oC |
| KspI | ...CCGC GG...
...GG CGCC... | Kluyvera species | 37 oC |
| Ksp22I | ...T GATCA...
...ACTAG T... | Kurthia species | 37 oC |
| Ksp632I | ...CTCTTCN NNNN...
...GAGAAGNNNN N... | Kluyvera species | 37 oC |
| KspAI | ...GTT AAC...
...CAA TTG... | Kurthia species | 37 oC |
| Kzo9I | ...N GATCN...
...NCTAG N... | Kurthia zopfii | 37 oC |
| LweI | ...GCATCNNNNN NNNNN...
...CGTAGNNNNNNNNN N... | Listeria welshimeri | 37 oC |
