| Enzyme | Restriction site | Origin | Temperature |
|---|
| TaaI | ...ACN GT...
...TG NCA... | Thermus aquaticus | 65 oC |
| TaiI | ...NACGT N...
...N TGCAN... | Thermus aquaticus | 65 oC |
| TaqI | ...T CGA...
...AGC T... | Thermus aquaticus | 65 oC |
| TasI | ...N AATTN...
...NTTAA N... | Thermus aquaticus | 65 oC |
| TatI | ...W GTACW...
...WCATG W... | Thermus aquaticus | 65 oC |
| TauI | ...GCSG C...
...C GSCG... | Thermus aquaticus | 55 oC |
| TfiI | ...G AWTC...
...CTWA G... | Thermus filiformis | 65 oC |
| TliI | ...C TCGAG...
...GAGCT C... | Thermococcus litoralis | 75 oC |
| Tru1I | ...T TAA...
...AAT T... | Meiothermus ruber | 65 oC |
| Tru9I | ...T TAA...
...AAT T... | Meiothermus ruber | 65 oC |
| TseI | ...G CWGC...
...CGWC G... | Thermus species | 65 oC |
| Tsp45I | ...N GTSACN...
...NCASTG N... | Thermus species | 65 oC |
| Tsp509I | ...N AATTN...
...NTTAA N... | Thermus species | 65 oC |
| TspDTI | ...ATGAANNNNNNNNNNN N...
...TACTTNNNNNNNNN NNN... | Thermus species | 70 oC |
| TspGWI | ...ACGGANNNNNNNNNNN N...
...TGCCTNNNNNNNNN NNN... | Thermus species | 70 oC |
| TspRI | ...NNNCASTGNN N...
...N NNGASACNNN... | Thermus species | 65 oC |
| Tth111I | ...GACN NNGTC...
...CTGNN NCAG... | Thermus thermophilus | 65 oC |
| Van91I | ...CCANNNN NTGG...
...GGTN NNNNACC... | Listonella anguillarum | 37 oC |
| Vha464I | ...C TTAAG...
...GAATT C... | Vibrio harveyi | 37 oC |
| VneI | ...G TGCAC...
...CACGT G... | Vibrio nereis | 37 oC |
| VpaK11BI | ...G GWCC...
...CCWG G... | Vibrio parahaemolyticus | 30 oC |
| VspI | ...AT TAAT...
...TAAT TA... | Vibrio species | 37 oC |
| XagI | ...CCTNN NNNAGG...
...GGANNN NNTCC... | Xanthobacter agilis | 37 oC |
| XapI | ...R AATTY...
...YTTAA R... | Xylophilus ampelinus | 37 oC |
| XbaI | ...T CTAGA...
...AGATC T... | Xanthomonas campestris | 37 oC |
| XceI | ...RCATG Y...
...Y GTACR... | Xanthomonas campestris | 37 oC |
| XcmI | ...CCANNNNN NNNNTGG...
...GGTNNNN NNNNNACC... | Xanthomonas campestris | 37 oC |
| XhoI | ...C TCGAG...
...GAGCT C... | Xanthomonas campestris | 37 oC |
| XhoII | ...R GATCY...
...YCTAG R... | Xanthomonas campestris | 37 oC |
| XmaI | ...C CCGGG...
...GGGCC C... | Xanthomonas campestris | 37 oC |
| XmaCI | ...C CCGGG...
...GGGCC C... | Xanthomonas campestris | 37 oC |
| XmaJI | ...C CTAGG...
...GGATC C... | Stenotrophomonas maltophilia | 37 oC |
| XmiI | ...GT MKAC...
...CAKM TG... | Stenotrophomonas maltophilia | 37 oC |
| XmnI | ...GAANN NNTTC...
...CTTNN NNAAG... | Xanthomonas axonopodis | 37 oC |
| XspI | ...C TAG...
...GAT C... | Xanthomonas species | 37 oC |
| ZraI | ...GAC GTC...
...CTG CAG... | Zoogloea ramigera | 37 oC |
| ZrmI | ...AGT ACT...
...TCA TGA... | Zoogloea ramigera | 37 oC |
| Zsp2I | ...ATGCA T...
...T ACGTA... | Zoogloea species | 37 oC |
